Anticorps Monoclonal anti-GATA3

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugaison

Non conjugué

Publications(18)

CloneNo.

1B11A8

Avis (1)

N° de cat : 66400-1-Ig

Synonymes

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

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Galerie de données de validation

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  WB analysis of MCF-7 using 66400-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66400-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66400-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66400-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66400-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66400-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66400-1-Ig(GATA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules Jurkat, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

66400-1-Ig cible GATA3 dans les applications de WB, IHC, IF/ICC, IF-P, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag0174
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC003070
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.

Publications

Species	Application	Title
human	WB,IHC	Drug Resist Updat; GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells; Authors - Zhen Zhu
human	WB	Cardiovasc Diabetol; BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling; Authors - Xinyue Huang
pig	WB	J Ethnopharmacol; Therapeutic effects and mechanisms study of Hanchuan Zupa Granule in a Guinea pig model of cough variant asthma.; Authors - Pengfei Li
mouse	WB	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	WB	Int Arch Allergy Immunol; Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2 Cell Differentiation.; Authors - Yan Li
mouse	WB,IF	Evid Based Complement Alternat Med; Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway.; Authors - Zhaoyu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Renan (Verified Customer) (08-18-2022)

The fluorescence staining was noticeable, but the intensity was not so high, after using a tyramide signal amplification (TSA) protocol. The picture shows a lung lymphoid follicle, part of a 4-um FFPE tissue section. Heat-induced antigen retrieval was done with TRIS-EDTA buffer (pH 9.0) and GATA-3 was stained using a tyramide-conjugated Alexa Fluor 488 dye. CD3 is stained in yellow.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Lung lymphoid follicle

GATA3 Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in Lung lymphoid follicle (Cat no:66400-1-Ig)

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