Anticorps Monoclonal anti-GATA3

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B11A8

N° de cat : 66400-1-PBS

Synonymes

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

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Galerie de données de validation

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  WB analysis of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66400-1-Ig (same clone as 66400-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66400-1-Ig (same clone as 66400-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66400-1-Ig (same clone as 66400-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66400-1-Ig (same clone as 66400-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66400-1-Ig(GATA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66400-1-PBS cible GATA3 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag0174
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC003070
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.

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