Anticorps Recombinant de lapin anti-GATA3

GATA3 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240684A2

N° de cat : 83808-1-PBS

Synonymes

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

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Galerie de données de validation

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  WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83808-1-RR (same clone as 83808-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01933-1

  Sandwich ELISA standard curve of MP01933-1, Human GATA3 Recombinant Matched Antibody Pair - PBS only. 83808-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0174. 83808-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP01933-1

  The mean GATA3 concentration was determined to be 21.82 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

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  FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  FC experiment of U937 using 83808-1-RR (same clone as 83808-1-PBS)

  1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01933-1

  Cytometric bead array standard curve of MP01933-1, GATA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83808-1-PBS. Detection antibody: 83808-4-PBS. Standard: Ag0174. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83808-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

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Informations sur le produit

83808-1-PBS cible GATA3 dans les applications de WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag0174
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 37 kDa and 48 kDa
• Numéro d’acquisition GenBank: BC003070
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).

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