Anticorps Polyclonal de lapin anti-GATA5

GATA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 55433-1-AP

Synonymes

Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 55433-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55433-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 55433-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 55433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA5 antibody (55433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HepG2
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

55433-1-AP cible GATA5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GATA binding protein 5
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: NM_080473
• Symbole du gène: GATA5
• Identification du gène (NCBI): 140628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP	Cancers (Basel); Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.; Authors - Qinghua Wang
human,mouse	WB,IF,IP	Biochem Biophys Res Commun; Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways.; Authors - Anmin Ren
human	WB	Nat Commun; Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy; Authors - Wenjing Bai
mouse	WB	Int J Biol Sci; Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice; Authors - Qianqian Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kamal (Verified Customer) (01-26-2024)

Works well when diluted in 1X TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue

{{ptg:RelatedPrimaryAntibodies}}