Anticorps Recombinant de lapin anti-GBP1

GBP1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240634G8

N° de cat : 83751-6-RR

Synonymes

Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-

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Galerie de données de validation

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  WB analysis using 83751-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83751-6-RR (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83751-6-RR

  1x10^6 HeLa cells untreated (dashed lines) or treated with IFN gamma (red) were intracellularly stained with 0.25 ug GBP1 Recombinant antibody (83751-6-RR, Clone: 240634G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83751-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83751-6-RR against Human GBP1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HUVEC
• Résultats positifs en FC (Intra): IFN gamma (100ng/ml 24h) HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83751-6-RR cible GBP1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GBP1 Protéine recombinante Ag7562
• Nom complet: GTP binding protein 1
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC002666
• Symbole du gène: GBP1
• Identification du gène (NCBI): 2633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by interferon and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II interferons and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus, and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.

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