Anticorps Polyclonal de lapin anti-GBP2

• Phare
• Validé par KD/KO

GBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

43

Avis (1)

N° de cat : 11854-1-AP

Synonymes

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

---

Galerie de données de validation

• 

  WB analysis using 11854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11854-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11854-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11854-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11854-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11854-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 11854-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 11854-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11854-1-AP (GBP2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 11854-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11854-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11854-1-AP (GBP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBP2 antibody (11854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules A375, cellules K-562, tissu placentaire humain, tissu pulmonaire humain, tissu splénique de rat
• Résultats positifs en IHC: tissu splénique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 32 publications below
• IHC: See 9 publications below
• IF: See 17 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11854-1-AP cible GBP2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GBP2 Protéine recombinante Ag2425
• Nom complet: guanylate binding protein 2, interferon-inducible
• Masse moléculaire calculée: 591 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC022272
• Symbole du gène: GBP2
• Identification du gène (NCBI): 2634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness, because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

Publications

Species	Application	Title
human	WB,IF	Nature; Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence.; Authors - Peng Li
mouse	WB,IHC,IF	Nature; Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.; Authors - Etienne Meunier
mouse	WB	Cell; IRF8 Regulates Transcription of Naips for NLRC4 Inflammasome Activation.; Authors - Rajendra Karki
human	WB	Cell; IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.; Authors - Si Ming Man
mouse	WB	Nat Immunol; The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection.; Authors - Si Ming Man
mouse	WB,IF	Nat Immunol; Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.; Authors - Etienne Meunier ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kyosuke (Verified Customer) (06-12-2019)

This antibody works very well for WB using HUVECs.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs

Produits associés

GBP1 Polyclonal antibody

15303-1-AP

P53 Polyclonal antibody

10442-1-AP

GBP5 Polyclonal antibody

13220-1-AP

SRPK1 Polyclonal antibody

14073-1-AP

EIF2AK2, PKR Polyclonal antibody

18244-1-AP

MX1 Polyclonal antibody

13750-1-AP

OAS1/3 Polyclonal antibody

14955-1-AP

OAS3 Polyclonal antibody

21915-1-AP