Anticorps Polyclonal de lapin anti-GBP4

GBP4 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17746-1-AP

Synonymes

Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4

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Galerie de données de validation

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  WB analysis using 17746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17746-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17746-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17746-1-AP

  IP result of anti-GBP4 (IP:17746-1-AP, 4ug; Detection:17746-1-AP 1:500) with A431 cells lysate 1040 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, tissu cardiaque de souris, tissu placentaire humain
• Résultats positifs en IP: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17746-1-AP cible GBP4 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GBP4 Protéine recombinante Ag12006
• Nom complet: guanylate binding protein 4
• Masse moléculaire calculée: 640 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC070055
• Symbole du gène: GBP4
• Identification du gène (NCBI): 115361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

Publications

Species	Application	Title
human	WB	mBio; Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation; Authors - Antonia R Bass
		PLoS One; Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner; Authors - Tomozumi Imamichi

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