Anticorps Polyclonal de lapin anti-GBX2

• Phare
• Validé par KD/KO

GBX2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 21639-1-PBS

Synonymes

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2

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Galerie de données de validation

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  WB analysis of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

  WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse stomach using 21639-1-AP (same clone as 21639-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

  IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21639-1-PBS cible GBX2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GBX2 Protéine recombinante Ag16288
• Nom complet: gastrulation brain homeobox 2
• Masse moléculaire calculée: 348 aa, 37 kDa
• Poids moléculaire observé: 33-37 kDa
• Numéro d’acquisition GenBank: BC137448
• Symbole du gène: GBX2
• Identification du gène (NCBI): 2637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

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