Anticorps Polyclonal de lapin anti-GCLC

• Phare
• Validé par KD/KO

GCLC Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 12601-1-PBS

Synonymes

EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC

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Galerie de données de validation

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  WB analysis using 12601-1-AP (same clone as 12601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 12601-1-AP (same clone as 12601-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12601-1-AP (same clone as 12601-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

  IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12601-1-PBS cible GCLC dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCLC Protéine recombinante Ag3252
• Nom complet: glutamate-cysteine ligase, catalytic subunit
• Masse moléculaire calculée: 637 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC022487
• Symbole du gène: GCLC
• Identification du gène (NCBI): 2729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

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