Anticorps Recombinant de lapin anti-GCLM

• Phare
• Validé par KD/KO

GCLM Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4K16

N° de cat : 82623-1-PBS

Synonymes

Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GLCLR, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit

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Galerie de données de validation

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  WB analysis using 82623-1-RR (same clone as 82623-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82623-1-RR (same clone as 82623-1-PBS)

  WB result of GCLM antibody (82623-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GCLM transfected HeLa cells. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 82623-1-RR (same clone as 82623-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82623-1-RR (GCLM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82623-1-RR (GCLM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 82623-1-RR (same clone as 82623-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82623-1-RR (GCLM antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82623-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82623-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82623-1-RR against Human GCLM were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82623-1-PBS cible GCLM dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GCLM Protéine recombinante Ag5497
• Nom complet: glutamate-cysteine ligase, modifier subunit
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC041809
• Symbole du gène: GCLM
• Identification du gène (NCBI): 2730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the first step of GSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

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