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Anticorps Recombinant de lapin anti-GCNA

GCNA Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241428A9

N° de cat : 84124-4-PBS

Synonymes

ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using GCNA Recombinant antibody (84124-4-RR)

    WB analysis using 84124-4-RR (same clone as 84124-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84124-4-RR (GCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84124-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GCNA Recombinant antibody (84124-4-RR)

    IF Staining of LNCaP using 84124-4-RR (same clone as 84124-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using GCNA antibody (84124-4-RR, Clone: 241428A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84124-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84124-4-RR

    Affinity and Kinetic Characterization of 84124-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84124-4-RR against Human GCNA were performed. The affinity constant is below 1 pM.

Informations sur le produit

84124-4-PBS cible GCNA dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène GCNA Protéine recombinante Ag21895
Nom complet acidic repeat containing
Masse moléculaire calculée 691 aa, 76 kDa
Poids moléculaire observé90 kDa
Numéro d’acquisition GenBankBC136259
Symbole du gène GCNA
Identification du gène (NCBI) 93953
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).

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