Anticorps Polyclonal de lapin anti-GCSH

• Phare
• Validé par KD/KO

GCSH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 16726-1-AP

Synonymes

GCE, GCSH, NKH

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Galerie de données de validation

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  WB analysis of HEK-293 using 16726-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16726-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16726-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16726-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16726-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16726-1-AP

  IP Result of anti-GCSH (IP:16726-1-AP, 3ug; Detection:16726-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50.

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  IHC staining of rat ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50.

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  IHC staining of human placenta using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 16726-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 16726-1-AP

  Immunofluorescent analysis of HepG2 cells, using GCSH antibody 16726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de souris, tissu hépatique humain, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cutané humain, tissu hépatique humain, tissu ovarien de rat, tissu ovarien humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16726-1-AP cible GCSH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCSH Protéine recombinante Ag10174
• Nom complet: glycine cleavage system protein H (aminomethyl carrier)
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC000790
• Symbole du gène: GCSH
• Identification du gène (NCBI): 2653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Publications

Species	Application	Title
		Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia ; KD Validated
human	WB	J Biol Chem; Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.; Authors - Daniel R Crooks
human	WB,IHC,IF	Sci Rep; GCSH antisense regulation determines breast cancer cells' viability.; Authors - Anna Adamus
human	WB	J Biol Chem; FDX1 regulates cellular protein lipoylation through direct binding to LIAS; Authors - Margaret B Dreishpoon
human	WB	PLoS One; Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.; Authors - Mark A Johnson