Anticorps Polyclonal de lapin anti-GCSH

• Phare
• Validé par KD/KO

GCSH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16726-1-PBS

Synonymes

NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE

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Galerie de données de validation

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  WB analysis of HEK-293 using 16726-1-AP (same clone as 16726-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 16726-1-AP (same clone as 16726-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 16726-1-AP (same clone as 16726-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16726-1-AP (same clone as 16726-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 16726-1-AP (same clone as 16726-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 16726-1-AP (same clone as 16726-1-PBS)

  IP result of anti-GCSH (IP:16726-1-AP, 3ug; Detection:16726-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16726-1-AP (same clone as 16726-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16726-1-AP (same clone as 16726-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using GCSH antibody 16726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16726-1-PBS cible GCSH dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCSH Protéine recombinante Ag10174
• Nom complet: glycine cleavage system protein H (aminomethyl carrier)
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC000790
• Symbole du gène: GCSH
• Identification du gène (NCBI): 2653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

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