Anticorps Polyclonal de lapin anti-GDAP1

GDAP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 13152-1-AP

Synonymes

CMTRIA, Ganglioside-induced differentiation-associated protein 1

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Galerie de données de validation

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  WB analysis using 13152-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 13152-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse brain using 13152-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of human brain using 13152-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13152-1-AP cible GDAP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GDAP1 Protéine recombinante Ag3769
• Nom complet: ganglioside-induced differentiation-associated protein 1
• Masse moléculaire calculée: 358 aa, 41 kDa
• Poids moléculaire observé: 36-41 kDa
• Numéro d’acquisition GenBank: BC024939
• Symbole du gène: GDAP1
• Identification du gène (NCBI): 54332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

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