Anticorps Recombinant de lapin anti-GDF-15

GDF-15 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242173B11

N° de cat : 85100-1-RR

Synonymes

GDF15, MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF 15

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Galerie de données de validation

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  WB analysis using 85100-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85100-1-RR (GDF15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85100-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85100-1-RR (GDF-15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 85100-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85100-1-RR (GDF-15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 85100-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85100-1-RR (GDF-15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85100-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85100-1-RR against Human GDF-15 were performed. The affinity constant is 39.3 nM.

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules A549, cellules HepG2, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85100-1-RR cible GDF-15 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GDF-15 Protéine recombinante Ag26790
• Nom complet: growth differentiation factor 15
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC008962
• Symbole du gène: GDF15
• Identification du gène (NCBI): 9518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

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