- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-GDF-15
GDF-15 Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 27455-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HT-1080, cellules HepG2, cellules LNCaP, tissu placentaire humain |
Résultats positifs en IHC | tissu placentaire humain, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 16 publications below |
WB | See 42 publications below |
IHC | See 8 publications below |
IF | See 4 publications below |
Informations sur le produit
27455-1-AP cible GDF-15 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | Humain |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | GDF-15 Protéine recombinante Ag26760 |
Nom complet | growth differentiation factor 15 |
Masse moléculaire calculée | 34 kDa |
Poids moléculaire observé | 34 kDa |
Numéro d’acquisition GenBank | BC008962 |
Symbole du gène | GDF15 |
Identification du gène (NCBI) | 9518 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.
Protocole
Product Specific Protocols | |
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WB protocol for GDF-15 antibody 27455-1-AP | Download protocol |
IHC protocol for GDF-15 antibody 27455-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer. | ||
Cancers (Basel) GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.
| ||
Commun Biol Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation | ||
J Cell Physiol Tumor-Derived GDF15 Induces Tumor Associated Fibroblast Transformation From BMSCs and Fibroblasts in Oral Squamous Cell Carcinoma
| ||
Mol Neurobiol Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation | ||
Antioxidants (Basel) Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Anna (Verified Customer) (08-01-2025) | Working nicely for Western Blot applications using a dilution of 1:5000.
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