• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-GDF-15

GDF-15 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(36)

N° de cat : 27455-1-AP

Synonymes

GDF15, GDF 15, growth differentiation factor 15, Growth/differentiation factor 15, MIC-1



Applications testées

Résultats positifs en WBcellules HT-1080, cellules HepG2, cellules LNCaP, tissu placentaire humain
Résultats positifs en IHCtissu placentaire humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

27455-1-AP cible GDF-15 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GDF-15 Protéine recombinante Ag26760
Nom complet growth differentiation factor 15
Masse moléculaire calculée 34 kDa
Poids moléculaire observé 34 kDa
Numéro d’acquisition GenBankBC008962
Symbole du gène GDF15
Identification du gène (NCBI) 9518
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Protocole

Product Specific Protocols
WB protocol for GDF-15 antibody 27455-1-APDownload protocol
IHC protocol for GDF-15 antibody 27455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.

Authors - Tiziano Bernasocchi
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
  • KD Validated
humanWB

Commun Biol

Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation

Authors - Guang Yang
humanWB

J Cell Physiol

Tumor-Derived GDF15 Induces Tumor Associated Fibroblast Transformation From BMSCs and Fibroblasts in Oral Squamous Cell Carcinoma

Authors - Jingjing Zhao
  • KD Validated
humanWB

Mol Neurobiol

Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation

Authors - Ruicheng Yang
humanWB

Antioxidants (Basel)

Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.

Authors - Lihua Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Anna (Verified Customer) (08-01-2025)

Working nicely for Western Blot applications using a dilution of 1:5000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human
{{ptg:RelatedPrimaryAntibodies}}