Anticorps Monoclonal anti-GDF-15

GDF-15 Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

1B1H4

N° de cat : 68768-1-PBS

Synonymes

GDF15, 1B1H4, GDF 15, growth differentiation factor 15, Growth/differentiation factor 15

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Galerie de données de validation

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  WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

  Untreated and Brefeldin A treated LNCaP cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  WB analysis of Caki-1 using 68768-1-Ig (same clone as 68768-1-PBS)

  Caki-1 cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50133-1

  Sandwich ELISA standard curve of MP50133-1, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-3-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-2

  Sandwich ELISA standard curve of MP50133-2, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-4-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-4

  Sandwich ELISA standard curve of MP50133-4, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-7-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-5

  Sandwich ELISA standard curve of MP50133-5, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-1-PBS. Detection antibody: HRP-conjugated 68768-2-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-8

  Sandwich ELISA standard curve of MP50133-8, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-5-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-9

  Sandwich ELISA standard curve of MP50133-9, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-6-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sample test of MP50133-1

  Cytometric bead array of cell line supernatant using MP50133-1, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-3-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: HepG2, LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-2

  Cytometric bead array of cell line supernatant using MP50133-2, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-4-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: HepG2, LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-4

  Cytometric bead array of cell line supernatant using MP50133-4, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-7-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-5

  Cytometric bead array of cell line supernatant using MP50133-5, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-1-PBS. Detection antibody: 68768-2-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-8

  Cytometric bead array of LnCap cells supernatant using MP50133-8, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-5-PBS. Detection antibody: 68768-1-PBS. Complete culture medium was served as blank control.

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  Sample test of MP50133-9

  Cytometric bead array of cell line supernatant using MP50133-9, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-6-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LnCap, HT-1080. Complete culture medium was served as blank control.

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  IHC staining of human placenta using 68768-1-Ig (same clone as 68768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68768-1-Ig (GDF-15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68768-1-Ig (same clone as 68768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68768-1-Ig (GDF-15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68768-1-Ig (same clone as 68768-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GDF15 antibody (68768-1-Ig, Clone: 1B1H4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68768-1-PBS cible GDF-15 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GDF-15 Protéine recombinante Ag33750
• Nom complet: growth differentiation factor 15
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC008962
• Symbole du gène: GDF15
• Identification du gène (NCBI): 9518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

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