Anticorps Polyclonal de lapin anti-BMP9

BMP9 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17769-1-AP

Synonymes

GDF2, GDF-2, GDF 2, Bone morphogenetic protein 9, BMP-9

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Galerie de données de validation

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  WB analysis of L02 using 17769-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 17769-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 17769-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules SMMC-7721
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

17769-1-AP cible BMP9 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMP9 Protéine recombinante Ag12177
• Nom complet: growth differentiation factor 2
• Masse moléculaire calculée: 429 aa, 47 kDa
• Poids moléculaire observé: 30 kDa, 46 kDa
• Numéro d’acquisition GenBank: BC074921
• Symbole du gène: BMP9
• Identification du gène (NCBI): 2658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. Human BMP9 precursor protein contains 429 amino acids and has a calculated molecular mass of about 47 kD. Posttranslational processing results in a mature BMP9 protein of about 13 kD. Once secreted, BMP9 may exist as dimers of mature regions or as BMP9 proregion complexes in which the proregion of BMP9 remains tightly associated with the mature dimer.

Publications

Species	Application	Title
human	WB	Heliyon; CHRDL2 activates the PI3K/AKT pathway to ameliorate glucocorticoid-induced damages to bone microvascular endothelial cells (BMECs); Authors - Xianzhe Huang
rat	WB	Int Immunopharmacol; Exosomal miR-574-3p from adipose-derived mesenchymal stem modulates CRIM1/BMPs signaling to restrain chondrocytes hypertrophy and inflammatory response in knee osteoarthritis; Authors - Junfeng Kang
mouse	WB	Geroscience; Tilorone attenuates high-fat diet-induced hepatic steatosis by enhancing BMP9-Smad1/5/8 signaling; Authors - Barnabas Horvath

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