Anticorps Polyclonal de lapin anti-GDF8/Myostatin

• Phare
• Validé par KD/KO

GDF8/Myostatin Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19142-1-PBS

Synonymes

GDF8, MSTN, myostatin, GDF 8, GDF-8

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Galerie de données de validation

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  WB analysis using 19142-1-AP (same clone as 19142-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 19142-1-AP (same clone as 19142-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 19142-1-AP (same clone as 19142-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 19142-1-AP (same clone as 19142-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19142-1-PBS cible GDF8/Myostatin dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GDF8/Myostatin Protéine recombinante Ag13605
• Nom complet: myostatin
• Masse moléculaire calculée: 375 aa, 43 kDa
• Poids moléculaire observé: 50 and 35 kDa
• Numéro d’acquisition GenBank: BC074757
• Symbole du gène: MSTN
• Identification du gène (NCBI): 2660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997). GDF8 may have cross-reactions with GDF11.

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