Anticorps Monoclonal anti-GDI1

GDI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E1G1

N° de cat : 66434-1-PBS

Synonymes

OPHN2, RABGDIA, XAP4, MRX41, GDP dissociation inhibitor 1

---

Galerie de données de validation

• 

  WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66434-1-Ig (same clone as 66434-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66434-1-Ig (same clone as 66434-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66434-1-Ig (same clone as 66434-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66434-1-Ig (same clone as 66434-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66434-1-PBS cible GDI1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GDI1 Protéine recombinante Ag17511
• Nom complet: GDP dissociation inhibitor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000317
• Symbole du gène: GDI1
• Identification du gène (NCBI): 2664
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)

{{ptg:RelatedPrimaryAntibodies}}