Anticorps Polyclonal de lapin anti-GDI2

GDI2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 10116-1-AP

Synonymes

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, Rab GDI β, RABGDIB

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Galerie de données de validation

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  WB analysis of A431 using 10116-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10116-1-AP (GDI2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10116-1-AP (GDI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

10116-1-AP cible GDI2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, levure, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GDI2 Protéine recombinante Ag0170
• Nom complet: GDP dissociation inhibitor 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC005145
• Symbole du gène: GDI2
• Identification du gène (NCBI): 2665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

Publications

Species	Application	Title
monkey	WB	Science; A bifunctional bacterial protein links GDI displacement to Rab1 activation.; Authors - Machner Matthias P MP
yeast	WB	Proc Natl Acad Sci U S A; Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA.; Authors - Zhu Yongqun Y
mouse	IF	Curr Biol; Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.; Authors - Anil Kumar Ganga
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
mouse	WB	Mol Cell Proteomics; Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene.; Authors - Wishart Thomas M TM
human	IHC	Endocr Relat Cancer; Comprehensive gene expression profiling of anaplastic thyroid cancers with cDNA microarray of 25 344 genes.; Authors - Onda M M

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guo-rong (Verified Customer) (08-30-2022)

Specific and clear results

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ARPE-19

GDI2 Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:10116-1-AP)