Anticorps Monoclonal anti-GEFT

GEFT Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5A11F8

N° de cat : 66012-1-PBS

Synonymes

ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66012-1-Ig (same clone as 66012-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66012-1-Ig (same clone as 66012-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEFT antibody (66012-1-Ig, Clone: 5A11F8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66012-1-PBS cible GEFT dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GEFT Protéine recombinante Ag10819
• Nom complet: RhoA/RAC/CDC42 exchange factor
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 53 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC012860
• Symbole du gène: GEFT
• Identification du gène (NCBI): 115557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).

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