Anticorps Polyclonal de lapin anti-GEMIN5

• Phare
• Validé par KD/KO

GEMIN5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24897-1-PBS

Synonymes

Gem associated protein 5, Gem-associated protein 5

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Galerie de données de validation

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  WB analysis using 24897-1-AP (same clone as 24897-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24897-1-AP (same clone as 24897-1-PBS)

  WB result of GEMIN5 antibody (24897-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

  IP result of anti-GEMIN5 (IP:24897-1-AP, 4ug; Detection:24897-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

  Immunofluorescent analysis of HeLa cells using 24897-1-AP (GEMIN5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24897-1-PBS cible GEMIN5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GEMIN5 Protéine recombinante Ag19268
• Nom complet: gem (nuclear organelle) associated protein 5
• Masse moléculaire calculée: 1508 aa, 169 kDa
• Poids moléculaire observé: 168 kDa
• Numéro d’acquisition GenBank: BC113614
• Symbole du gène: GEMIN5
• Identification du gène (NCBI): 25929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

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