Anticorps Recombinant de lapin anti-GEMIN5

• Phare
• Validé par KD/KO

GEMIN5 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241205G7

N° de cat : 84171-5-RR

Synonymes

241205G7, Gem associated protein 5, Gem-associated protein 5

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Galerie de données de validation

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  WB analysis of HEK-293 using 84171-5-RR

  WB result of GEMIN5 antibody (84171-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells.

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  WB analysis using 84171-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84171-5-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 84171-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84171-5-RR (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 84171-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84171-5-RR (GEMIN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84171-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-5-RR, Clone: 241205G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HEK-293 using 84171-5-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GEMIN5 Recombinant antibody (84171-5-RR, Clone:241205G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84171-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84171-5-RR against Human GEMIN5 were performed. The affinity constant is 0.619 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules COLO 320, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84171-5-RR cible GEMIN5 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GEMIN5 Protéine recombinante Ag19220
• Nom complet: gem (nuclear organelle) associated protein 5
• Masse moléculaire calculée: 1508 aa, 169 kDa
• Poids moléculaire observé: 168-180 kDa
• Numéro d’acquisition GenBank: BC113614
• Symbole du gène: GEMIN5
• Identification du gène (NCBI): 25929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

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