Anticorps Polyclonal de lapin anti-GEMIN8

GEMIN8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15251-1-PBS

Synonymes

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1

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Galerie de données de validation

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  WB analysis using 15251-1-AP (same clone as 15251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15251-1-AP (same clone as 15251-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

  IP result of anti-GEMIN8 (IP:15251-1-AP, 3ug; Detection:15251-1-AP 1:400) with HepG2 cells lysate 1720ug. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15251-1-PBS cible GEMIN8 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GEMIN8 Protéine recombinante Ag6774
• Nom complet: gem (nuclear organelle) associated protein 8
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC003607
• Symbole du gène: GEMIN8
• Identification du gène (NCBI): 54960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.

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