Anticorps Monoclonal anti-GEN1

• Phare
• Validé par KD/KO

GEN1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A10D10

N° de cat : 68060-1-PBS

Synonymes

Gen, 1A10D10

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Galerie de données de validation

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  WB analysis of U2OS using 68060-1-Ig (same clone as 68060-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68060-1-Ig (same clone as 68060-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68060-1-Ig (same clone as 68060-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68060-1-Ig (same clone as 68060-1-PBS)

  WB result of GEN1 antibody (68060-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEN1 transfected A549 cells. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68060-1-PBS cible GEN1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GEN1 Protéine recombinante Ag31247
• Nom complet: Gen homolog 1, endonuclease (Drosophila)
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 98-100 kDa
• Numéro d’acquisition GenBank: NM_182625
• Symbole du gène: GEN1
• Identification du gène (NCBI): 348654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.

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