Anticorps Monoclonal anti-GFAP
GFAP Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, Lapin, porc, rat, souris
Applications
WB, IHC, IF-P, IP, ELISA
Conjugaison
Non conjugué
CloneNo.
4B2E10
N° de cat : 60190-1-PBS
Synonymes
Galerie de données de validation
Informations sur le produit
60190-1-PBS cible GFAP dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris
Réactivité | Humain, Lapin, porc, rat, souris |
Hôte / Isotype | Mouse / IgG2a |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | GFAP Protéine recombinante Ag10452 |
Nom complet | glial fibrillary acidic protein |
Masse moléculaire calculée | 432 aa, 50 kDa |
Poids moléculaire observé | 45-52 kDa |
Numéro d’acquisition GenBank | BC013596 |
Symbole du gène | GFAP |
Identification du gène (NCBI) | 2670 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS only |
Conditions de stockage | Store at -80°C. 20ul contiennent 0,1% de BSA. |
Informations générales
Function
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.Tissue specificity
GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).Involvement in disease
- Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
- Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.
Isoforms
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.