Anticorps Monoclonal anti-GFAP

GFAP Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4B2E10

N° de cat : CL594-60190

Synonymes

FLJ45472, GFAP



Applications testées

Résultats positifs en IFtissu cérébral de souris,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Applications publiées

IFSee 1 publications below

Informations sur le produit

CL594-60190 cible GFAP dans les applications de IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéesouris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène GFAP Protéine recombinante Ag10452
Nom complet glial fibrillary acidic protein
Masse moléculaire calculée 432 aa, 50 kDa
Numéro d’acquisition GenBankBC013596
Symbole du gène GFAP
Identification du gène (NCBI) 2670
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GFAP Function GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis. Tissue specificity GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963). Involvement in disease Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function. Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. Isoforms Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes. Post-translational modifications Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963). Cellular localization GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocole

Product Specific Protocols
IF protocol for CL594 GFAP antibody CL594-60190Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Glia

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes

Authors - Yue Wan