Anticorps Polyclonal de lapin anti-GFM1

GFM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14274-1-PBS

Synonymes

COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt

---

Galerie de données de validation

• 

  WB analysis using 14274-1-AP (same clone as 14274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14274-1-PBS cible GFM1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFM1 Protéine recombinante Ag5616
• Nom complet: G elongation factor, mitochondrial 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC049210
• Symbole du gène: GFM1
• Identification du gène (NCBI): 85476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]

{{ptg:RelatedPrimaryAntibodies}}