Anticorps Monoclonal anti-GFP tag

GFP tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1E10H7

N° de cat : 66002-1-PBS

Synonymes

eGFP, GFP, CFP, eYFP, GFP-tag

---

Galerie de données de validation

• 

  WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

  HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

  WB result of eGFP fusion protein with anti-eGFP tag (66002-1-Ig) with GFP fusion protein at varous dilutions. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Transfected HEK-293T using 66002-1-Ig (same clone as 66002-1-PBS)

  IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:10000) with Transfected HEK-293T cells lysate 400 ug. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66002-1-PBS cible GFP tag dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GFP tag Protéine recombinante Ag2128
• Nom complet: GFP tag
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: M62653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.

{{ptg:RelatedPrimaryAntibodies}}