Anticorps Recombinant de lapin anti-GFP tag

GFP tag Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante, Aequorea Victoria

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241614B3

N° de cat : 81835-2-RR

Synonymes

241614B3

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Galerie de données de validation

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  WB analysis of Recombinant protein using 81835-2-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 81835-2-RR

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected COS-7 using 81835-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected COS-7 cells using GFP tag antibody (81835-2-RR, Clone: 241614B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81835-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81835-2-RR against Aequorea victoria GFP tag were performed. The affinity constant is 0.619 nM.

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Applications testées

• Résultats positifs en WB: Protéine recombinante, cellules HEK-293 transfectées
• Résultats positifs en IF/ICC: Transfected COS-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81835-2-RR cible GFP tag dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Protéine recombinante, Aequorea Victoria

• Réactivité: Protéine recombinante, Aequorea Victoria
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GFP tag Protéine recombinante Ag2128
• Nom complet: GFP tag
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: M62653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

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