Anticorps Polyclonal de lapin anti-GFP tag

GFP tag Polyclonal Antibody for IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante, Aequorea Victoria

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-50430

Synonymes

eGFP, GFP, green, GFP-tag, eYFP

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Galerie de données de validation

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  IF Staining of mouse brain using CL488-50430

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFP tag antibody (CL488-50430) at dilution of 1:200, GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using CL488-50430

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFP tag antibody (CL488-50430) at dilution of 1:200, GFAP antibody (60190-1-Ig, Clone: 4B2E10, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-50430 cible GFP tag dans les applications de IF-P et montre une réactivité avec des échantillons Protéine recombinante, Aequorea Victoria

• Réactivité: Protéine recombinante, Aequorea Victoria
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFP tag Protéine recombinante Ag2128
• Nom complet: GFP tag
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: M62653
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

This antibody is labeled with CoraLite Plus 488.

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