Anticorps Monoclonal anti-GFP tag

GFP tag Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Protéine recombinante, Aequorea Victoria

Applications

WB, IF, CoIP

Conjugaison

HRP

Publications(19)

CloneNo.

1E10H7

Avis (2)

N° de cat : HRP-66002

Synonymes

eGFP, GFP, CFP, eYFP, GFP-tag

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Galerie de données de validation

• 

  WB analysis of Recombinant protein using HRP-66002

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66002 (GFP tag Antibody) at dilution of 1:10000.

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Applications testées

• Résultats positifs en WB: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

HRP-66002 cible GFP tag dans les applications de WB, IF, CoIP et montre une réactivité avec des échantillons Protéine recombinante, Aequorea Victoria

• Réactivité: Protéine recombinante, Aequorea Victoria
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: GFP tag
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: -
• Numéro d’acquisition GenBank: M62653
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a HRP conjugated mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

Species	Application	Title
	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang
	WB,IF	PLoS Biol; Bridging integrator 1 fragment accelerates tau aggregation and propagation by enhancing clathrin-mediated endocytosis in mice; Authors - Xingyu Zhang
	WB	Mol Neurodegener; Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease.; Authors - Guoxin Zhang
		Elife; A picorna-like virus suppresses the N-end rule pathway to inhibit apoptosis.; Authors - Zhaowei Wang
	WB	Aging Cell; Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease; Authors - Guoxin Zhang
	WB	Elife; Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.; Authors - Xingyu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (09-11-2023)

It works well in our detection of GFP-tagged protein from Arabidopsis

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Arabidopsis

FH

Aamir (Verified Customer) (01-08-2020)

Worked fine for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 10000
• Cell Tissue Type: HEks

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