Anticorps Polyclonal de lapin anti-GFPT1

• Phare
• Validé par KD/KO

GFPT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14132-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 14132-1-AP (same clone as 14132-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14132-1-AP (same clone as 14132-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14132-1-AP (same clone as 14132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14132-1-AP (same clone as 14132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 14132-1-AP (same clone as 14132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 14132-1-AP (same clone as 14132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14132-1-AP (same clone as 14132-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14132-1-PBS cible GFPT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GFPT1 Protéine recombinante Ag5312
• Nom complet: glutamine-fructose-6-phosphate transaminase 1
• Masse moléculaire calculée: 681 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC045641
• Symbole du gène: GFPT1
• Identification du gène (NCBI): 2673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

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