Anticorps Recombinant de lapin anti-GFPT2

GFPT2 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251454E5

N° de cat : 86609-3-PBS

Synonymes

D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 86609-3-RR (same clone as 86609-3-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86609-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86609-3-RR against Human GFPT2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86609-3-PBS cible GFPT2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GFPT2 Protéine recombinante Ag7275
• Nom complet: glutamine-fructose-6-phosphate transaminase 2
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 75-77 kDa
• Numéro d’acquisition GenBank: BC000012
• Symbole du gène: GFPT2
• Identification du gène (NCBI): 9945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.

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