Anticorps Polyclonal de lapin anti-GGA1

• Phare
• Validé par KD/KO

GGA1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 25674-1-AP

Synonymes

GGA1

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Galerie de données de validation

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  WB analysis of HEK-293 using 25674-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25674-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25674-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IF Staining of HepG2 using 25674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25674-1-AP (GGA1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

25674-1-AP cible GGA1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGA1 Protéine recombinante Ag22614
• Nom complet: golgi associated, gamma adaptin ear containing, ARF binding protein 1
• Poids moléculaire observé: 70-72 kDa
• Numéro d’acquisition GenBank: BC044629
• Symbole du gène: GGA1
• Identification du gène (NCBI): 26088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
human	WB,IF	J Biol Chem; GGA1 interacts with the endosomal Na+/H+ exchanger NHE6 governing localization to the endosome compartment; Authors - Li Ma
human	WB	Cell Rep; Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells; Authors - Yuuki Obata ; KD Validated
mouse	WB	Environ Toxicol; Total flavonoids of Rhizoma drynariae improves tendon-bone healing for anterior cruciate ligament reconstruction in mice and promotes the osteogenic differentiation of bone mesenchymal stem cells by the ERR1/2-Gga1-TGF-β/MAPK pathway; Authors - Lei Han
human	WB	Sci Adv; ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication; Authors - Guy J Pearson

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 25674-1-AP (GGA1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

GGA1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:25674-1-AP)

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