Anticorps Polyclonal de lapin anti-GGA3

GGA3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 33639-1-PBS

Synonymes

ADP-ribosylation factor-binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, KIAA0154

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Galerie de données de validation

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  WB analysis using 33639-1-AP (same clone as 33639-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33639-1-AP (GGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33639-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 33639-1-AP (same clone as 33639-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GGA3 antibody (33639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 33639-1-PBS in a different storage buffer formulation.

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Informations sur le produit

33639-1-PBS cible GGA3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGA3 Protéine recombinante Ag38658
• Nom complet: golgi associated, gamma adaptin ear containing, ARF binding protein 3
• Masse moléculaire calculée: 78kDa，723aa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: NM_138619.3
• Symbole du gène: GGA3
• Identification du gène (NCBI): 23163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Golgi-associated, gamma-adaptin ear-containing, ARF-binding protein 3 (GGA3) is a monomeric clathrin adaptor that localizes predominantly to the trans-Golgi network (TGN) and endosomal membranes. Beyond classical cargo sorting, GGA3 participates in receptor recycling, retrograde transport and signal termination. Notably, caspase-3 cleaves GGA3 during apoptosis or cerebral ischaemia; loss of this adaptor stabilises β-secretase (BACE1), thereby increasing amyloid-β production and linking GGA3 to Alzheimer disease pathogenesis. Recent work further implicates GGA3 in oncogenic processes: in non-small-cell lung cancer it supports TrkA trafficking to the plasma membrane, prolonging Akt/ERK signalling and promoting cell survival.(PMID: 39267722, PMID: 37586201)

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