Anticorps Polyclonal de lapin anti-GGCT

GGCT Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16257-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16257-1-AP (same clone as 16257-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 16257-1-AP (same clone as 16257-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 16257-1-AP (same clone as 16257-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 16257-1-AP (same clone as 16257-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16257-1-AP (same clone as 16257-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16257-1-AP (GGCT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 16257-1-AP (same clone as 16257-1-PBS)

  IP result of anti-GGCT (IP:16257-1-AP, 4ug; Detection:16257-1-AP 1:300) with mouse kidney tissue lysate 3000 ug. This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 16257-1-AP (same clone as 16257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 16257-1-AP (same clone as 16257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16257-1-AP (GGCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16257-1-AP (same clone as 16257-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16257-1-AP (GGCT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16257-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16257-1-PBS cible GGCT dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGCT Protéine recombinante Ag9284
• Nom complet: gamma-glutamyl cyclotransferase
• Masse moléculaire calculée: 188 aa, 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC019243
• Symbole du gène: GGCT
• Identification du gène (NCBI): 79017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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