Anticorps Recombinant de lapin anti-GGGGS Linker

GGGGS Linker Recombinant Antibody for WB, IHC, IF/ICC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

WB, IHC, IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

242306C8

N° de cat : 98262-1-RR

Synonymes

G4S, G4S link, G4S Linker, GGGGS, GS Linker

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Galerie de données de validation

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  WB analysis using 98262-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 98262-1-RR (GGGGS Linker antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of Transfected HEK-293T using 98262-1-RR

  Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens).

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  IF Staining of Transfected HEK-293T using 98262-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Transfected CHO-K1 using 98262-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected CHO-K1 using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Transfected HEK-293T using 98262-1-RR

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98262-1-RR

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed

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  FC experiment of Transfected HEK-293T using 98262-1-RR

  1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed

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  Affinity and Kinetic Characterization of 98262-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98262-1-RR against scFv (single chain fragment variable) format with variable regions of heavy (VH) and light (VL) chains joined together by G4S linker were performed. The affinity constant is 3.87 nM.

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Applications testées

• Résultats positifs en WB: Transfected HEK-293T cells
• Résultats positifs en IHC: Transfected HEK-293T cells, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: Transfected HEK-293T cells, Transfected CHO-K1
• Résultats positifs en cytométrie: Transfected HEK-293T cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:40000-1:160000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

98262-1-RR cible GGGGS Linker dans les applications de WB, IHC, IF/ICC, FC et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GGGGS Linker
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.

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