Anticorps Recombinant de lapin anti-GGGGS Linker

GGGGS Linker Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

FC

Conjugaison

Biotin

CloneNo.

242306C8

N° de cat : Biotin-98262

Synonymes

G4S, G4S link, G4S Linker, GGGGS, GS Linker

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using Biotin-98262

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Biotin Plus Anti-GGGGS Linker Rabbit RecAb (Biotin-98262, Clone: 242306C8) and CoraLite®488-conjugated streptavidin. Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using Biotin-98262

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Biotin Plus Anti-GGGGS Linker Rabbit RecAb (Biotin-98262, Clone:242306C8) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-98262 cible GGGGS Linker dans les applications de FC et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GGGGS Linker
• Identification du gène (NCBI): 42
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404).

{{ptg:RelatedPrimaryAntibodies}}