Anticorps Polyclonal de lapin anti-GGH

GGH Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 13264-1-AP

Synonymes

Conjugase, EC:3.4.19.9, Gamma-glutamyl hydrolase, Gamma-Glu-X carboxypeptidase, GH

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Galerie de données de validation

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  WB analysis using 13264-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13264-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13264-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13264-1-AP (GGH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13264-1-AP

  IP result of anti-GGH (IP:13264-1-AP, 4ug; Detection:13264-1-AP 1:2000) with HepG2 cells lysate 1800 ug.

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  IHC staining of human kidney using 13264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13264-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13264-1-AP (GGH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HepG2, cellules HT-1080, cellules MCF-7
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13264-1-AP cible GGH dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GGH Protéine recombinante Ag3939
• Nom complet: gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
• Masse moléculaire calculée: 318 aa, 36 kDa
• Poids moléculaire observé: 30-37 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC025025
• Symbole du gène: GGH
• Identification du gène (NCBI): 8836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GGH (Gamma glutamyl hydrolase), also named as GH or Conjugase, is a key lysosomal enzyme involved in the metabolism of folic acid and in the action of antifolate drugs (PMID: 16945597). GGH catalyzes the removal of γ-linked polyglutamates from the intracellular folylpolyglutamates to yield folylmonoglutamate coenzymes (PMID: 9614206). The full-length protein has a calculated molecular mass of 36 kDa, contains four potential asparagine glycosylation sites, and was predicted to have a 24-amino-acid signal peptide (PMID: 8816764). GGH can form homodimer which contains two potential active sites (PMID: 16945597). Some bands can be detected by SDS-PAGE: 35-37kDa (full-length), 55kDa (glycosylated form) and 30-33kDa (signal peptide removed) (PMID: 8621474).

Publications

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
	WB	Front Pharmacol; Up-regulation of ABCG1 is associated with methotrexate resistance in acute lymphoblastic leukemia cells; Authors - Yao Chen
human	IHC,IF	Reprod Sci; The Identification of Gamma-Glutamyl Hydrolase in Uterine Corpus Endometrial Carcinoma: a Predictive Model and Machine Learning; Authors - Cheng Zhu
human	WB	Mol Med; Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer; Authors - Shuxuan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (04-04-2025)

Good antibody for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, kidney, and brain

GGH Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, kidney, and brain (Cat no:13264-1-AP)

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