Anticorps Recombinant de lapin anti-GGT1

GGT1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230428B5

N° de cat : 83304-1-PBS

Synonymes

230428B5, CD224, D22S672, D22S732, GGT

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Galerie de données de validation

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  WB analysis using 83304-1-RR (same clone as 83304-1-PBS)

  Human kidney tissue were subjected to SDS PAGE followed by western blot with 83304-1-RR (GGT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83304-1-RR (same clone as 83304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83304-1-RR (GGT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83304-1-RR (same clone as 83304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83304-1-RR (GGT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 83304-1-PBS

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 83304-1-PBS (GGT1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83304-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 83304-1-PBS against Human GGT1 was performed. The affinity constant is 1.86 nM.

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Applications testées

• Résultats positifs en IHC: Human Kidney(renal cell) Cancer, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83304-1-PBS cible GGT1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GGT1 Protéine recombinante Ag34209
• Nom complet: gamma-glutamyltransferase 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_013430
• Symbole du gène: GGT1
• Identification du gène (NCBI): 2678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GGT1 is a transpeptidase whose activity increases the availability of amino acids, primarily cysteine, for intracellular GSH synthesis. GGT1 also plays a critical role in maintaining GSH homeostasis and defense against oxidative stress. In liver and obstructive biliary diseases, circulating GGT1 activity has widely been used for diagnosis purposes, as well as an indicator of alcohol consumption. (PMID: 31151999)

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