Anticorps Polyclonal de lapin anti-GHITM

• Phare
• Validé par KD/KO

GHITM Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16296-1-AP

Synonymes

DERP2, GHITM, HSPC282, MICS1, My021, PTD010, TMBIM5, UNQ244/PRO281

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Galerie de données de validation

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  WB analysis of mouse liver using 16296-1-AP

  mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16296-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16296-1-AP

  WB results of GHITM antibody (16296-1-AP) with siRNA sample.

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  WB analysis of HeLa using 16296-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 16296-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16296-1-AP (GHITM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16296-1-AP (GHITM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16296-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16296-1-AP (GHITM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

16296-1-AP cible GHITM dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GHITM Protéine recombinante Ag9482
• Nom complet: growth hormone inducible transmembrane protein
• Masse moléculaire calculée: 345 aa, 37 kDa
• Poids moléculaire observé: 42 kDa, 25-27 kDa
• Numéro d’acquisition GenBank: BC010354
• Symbole du gène: GHITM
• Identification du gène (NCBI): 27069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein which localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa. The apparent molecular weight has been reported to be 42 kDa, the increased size in the protein may be due to post-translational modifications (PMID: 11416014; 16412389). GHITM can be cleaved into smaller forms of 23-27 kDa (PMID: 16412389; 18417609).

Publications

Species	Application	Title
human	WB	EMBO J; Regulation of mitochondrial proteostasis by the proton gradient; Authors - Maria Patron
human	WB	Cells; Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery.; Authors - Bruno Seitaj ; KO Validated
human	WB	Life Sci Alliance; TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathy.; Authors - Li Zhang
mouse	WB	Nat Commun; Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling; Authors - L. Orliaguet