Anticorps Recombinant de lapin anti-GHITM

GHITM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240541D3

N° de cat : 83548-3-PBS

Synonymes

240541D3, Dermal papilla-derived protein 2, DERP2, Growth hormone-inducible transmembrane protein, MICS1

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Galerie de données de validation

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  WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83548-3-RR (same clone as 83548-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00524-1

  Cytometric bead array standard curve of MP00524-1, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-2-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00524-2

  Cytometric bead array standard curve of MP00524-2, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-1-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83548-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

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Informations sur le produit

83548-3-PBS cible GHITM dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GHITM Protéine recombinante Ag35170
• Nom complet: growth hormone inducible transmembrane protein
• Masse moléculaire calculée: 345 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC010354
• Symbole du gène: GHITM
• Identification du gène (NCBI): 27069
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.

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