Anticorps Polyclonal de lapin anti-GIGYF2

• Phare
• Validé par KD/KO

GIGYF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 24790-1-AP

Synonymes

GIGYF2, GYF2, KIAA0642, PARK11, PERQ2, PERQ3, TNRC15

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Galerie de données de validation

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  WB analysis using 24790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 24790-1-AP

  IP Result of anti-GIGYF2 (IP:24790-1-AP, 4ug; Detection:24790-1-AP 1:2000) with Jurkat cells lysate 2000ug.

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  IHC staining of human breast cancer using 24790-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24790-1-AP (GIGYF2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24790-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24790-1-AP (GIGYF2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 24790-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24790-1-AP (GIGYF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

24790-1-AP cible GIGYF2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GIGYF2 Protéine recombinante Ag20329
• Nom complet: GRB10 interacting GYF protein 2
• Masse moléculaire calculée: 1320 aa, 152 kDa
• Poids moléculaire observé: 150-170 kDa
• Numéro d’acquisition GenBank: BC146775
• Symbole du gène: GIGYF2
• Identification du gène (NCBI): 26058
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human,mouse	WB,IF	Redox Biol; Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function; Authors - Fanglin Niu ; KD Validated
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB,IF	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
human	IP,WB	Cell Rep; Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress; Authors - Michelle Harreman Lehner ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parisa (Verified Customer) (09-26-2023)

Good qulity, we could see some other non-specific bands close to the predicted GIGYF2 size and we could tell which one is GIGYF2 using the KO cells. But the antibody has a good specificity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293