Anticorps Polyclonal de lapin anti-GINS2

• Phare
• Validé par KD/KO

GINS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 16247-1-AP

Synonymes

PSF2, CGI-122, DC5, GINS 2, HSPC037

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Galerie de données de validation

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  WB analysis of Jurkat using 16247-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16247-1-AP (PSF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16247-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16247-1-AP (PSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16247-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16247-1-AP (PSF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 16247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16247-1-AP (PSF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16247-1-AP (PSF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 16247-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSF2 antibody (16247-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of Jurkat using 16247-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Polyclonal antibody (16247-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Jurkat,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

16247-1-AP cible GINS2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GINS2 Protéine recombinante Ag9180
• Nom complet: GINS complex subunit 2 (Psf2 homolog)
• Masse moléculaire calculée: 185 aa, 21 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC010164
• Symbole du gène: GINS2
• Identification du gène (NCBI): 51659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	WB	J Cell Biol; LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.; Authors - Yilin Fan ; KD Validated
human	WB	Cell Rep; Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction.; Authors - Jiin-Tarng Wang
mouse	WB	Oncogene; Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality.; Authors - Radoslaw Szmyd
human	WB	Mol Cell Biol; A Role for USP7 in DNA Replication.; Authors - Jagannathan Madhav M
human	WB	J Biol Chem; TIMELESS suppresses the accumulation of aberrant CDC45-MCM2-7-GINS replicative helicase complexes on human chromatin.; Authors - Xiaohua Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SHENGYAN (Verified Customer) (01-30-2025)

The antibody works great in western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Has been tested in 293T, HaCaT, 143B, Psn1, H460 human cell lines

GINS2 Antibody Western Blot validation (1:2000 dilution) in Has been tested in 293T, HaCaT, 143B, Psn1, H460 human cell lines (Cat no:16247-1-AP)

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