Anticorps Recombinant de lapin anti-GINS2

GINS2 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241870E9

N° de cat : 84486-2-PBS

Synonymes

PSF2, 241870E9, CGI-122, DC5, GINS 2

---

Galerie de données de validation

• 

  WB analysis using 84486-2-RR (same clone as 84486-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84486-2-RR (GINS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84486-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84486-2-RR (same clone as 84486-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GINS2 antibody (84486-2-RR, Clone: 241870E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84486-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 84486-2-RR (same clone as 84486-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Recombinant antibody (84486-2-RR, Clone:241870E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84486-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01325-1

  Cytometric bead array standard curve of MP01325-1, GINS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84486-2-PBS. Detection antibody: 84486-1-PBS. Standard: Ag9180. Range: 0.313-20 ng/mL.

• 

  Cytometric bead array standard curve of MP01325-2

  Cytometric bead array standard curve of MP01325-2, GINS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84486-2-PBS. Detection antibody: 84486-3-PBS. Standard: Ag9180. Range: 0.313-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 84486-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84486-2-RR against Human GINS2 were performed. The affinity constant is 0.82 nM.

---

Informations sur le produit

84486-2-PBS cible GINS2 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GINS2 Protéine recombinante Ag9180
• Nom complet: GINS complex subunit 2 (Psf2 homolog)
• Masse moléculaire calculée: 185 aa, 21 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC010164
• Symbole du gène: GINS2
• Identification du gène (NCBI): 51659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

{{ptg:RelatedPrimaryAntibodies}}