Anticorps Recombinant de lapin anti-GIRK2

GIRK2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250605D1

N° de cat : 86016-1-RR

Synonymes

KCNJ6, BIR1, G protein-activated inward rectifier potassium channel 2, GIRK 2, GIRK-2

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Galerie de données de validation

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  WB analysis using 86016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86016-1-RR (KCNJ6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86016-1-RR (KCNJ6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86016-1-RR against Human GIRK2 were performed. The affinity constant is 0.372 nM.

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Applications testées

• Résultats positifs en WB: MG-63 cells, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86016-1-RR cible GIRK2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GIRK2 Protéine recombinante Ag16344
• Nom complet: potassium inwardly-rectifying channel, subfamily J, member 6
• Masse moléculaire calculée: 423 aa, 48 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC101547
• Symbole du gène: GIRK2
• Identification du gène (NCBI): 3763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GIRK2 (also known as Kir3.2), encoded by KCNJ6 gene, is a member of the G protein-coupled inwardly-rectifying potassium channel (GIRK, Kir3) family of inward rectifier potassium channels. GIRK channels are activated following stimulation of G protein-coupled receptors (PMID: 26422984). They play important roles in regulating cellular excitabilities in the heart and brain (PMID: 31043612). Mutations in KCNJ6 gene are associated with Keppen-Lubinsky Syndrome (PMID: 25620207).

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