Anticorps Recombinant de lapin anti-GITR/TNFRSF18

GITR/TNFRSF18 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF, FC

Conjugaison

Non conjugué

Publications(1)

CloneNo.

250666B12

N° de cat : 98466-3-RR

Synonymes

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98466-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse GITR/TNFRSF18 Rabbit RecAb (98466-3-RR, Clone: 250666B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD25/IL-2RA (PC61.5) (APC-65137, Clone: PC61.5). CD4+ cells were pre-gated for analysis. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98466-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse GITR/TNFRSF18 Rabbit RecAb (98466-3-RR, Clone:250666B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). CD4+ cells were pre-gated for analysis. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

98466-3-RR cible GITR/TNFRSF18 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GITR/TNFRSF18 Protéine recombinante Eg3695
• Nom complet: tumor necrosis factor receptor superfamily, member 18
• Masse moléculaire calculée: 25kDa
• Numéro d’acquisition GenBank: NM_009400.2
• Symbole du gène: CD357
• Identification du gène (NCBI): 21936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

Publications

Species	Application	Title
mouse	IF	Adv Healthc Mater; Engineered Hollow Nanocomplex Combining Photothermal and Antioxidant Strategies for Targeted Tregs Depletion and Potent Immune Activation in Tumor Immunotherapy; Authors - Qi Sun

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