Anticorps Polyclonal de lapin anti-Connexin 32

Connexin 32 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 10450-1-AP

Synonymes

Connexin-32, GJB1, CMTX, CMTX1, CX32

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 10450-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (Connexin 32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10450-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10450-1-AP (Connexin 32 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (Connexin 32 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10450-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (Connexin 32 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (Connexin 32 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10450-1-AP

  IP result of anti-Connexin 32 (IP:10450-1-AP, 4ug; Detection:10450-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (Connexin 32 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (Connexin 32 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10450-1-AP (Connexin 32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10450-1-AP (Connexin 32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A375, tissu cutané de souris, tissu hépatique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10450-1-AP cible Connexin 32 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Connexin 32 Protéine recombinante Ag0659
• Nom complet: gap junction protein, beta 1, 32kDa
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002805
• Symbole du gène: Connexin-32
• Identification du gène (NCBI): 2705
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Connexin 32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin 32 is the major gap junction protein of liver, and the expression is also found in the brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin 32 plays an important role in peripheral nerve. Defects in Connexin 32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. Connexin 32 protein can be detected as a 27-32 kDa monomer and a 48-54 kDa dimer (PMID: 8266101; 8613761; 8608591; 19265674; 17972320; 19845480)

Publications

Species	Application	Title
human	WB	Chemosphere; Organophosphate flame retardant TDCPP: A risk factor for renal cancer?; Authors - Xuan Zhou
human	WB	World J Gastroenterol; PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.; Authors - Xiao-Ming Liu
mouse	WB	Brain Res Bull; Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.; Authors - Chen Men
human	IHC	J Biomed Biotechnol; Ischemia alters the expression of connexins in the aged human brain.; Authors - Nakase Taizen T
human	IHC,IF	Histopathology; Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.; Authors - Wen-Qun Zhong
human	WB	Oncol Lett; Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.; Authors - Lihua Huang

{{ptg:RelatedPrimaryAntibodies}}