Anticorps Polyclonal de lapin anti-Connexin-32

Connexin-32 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10450-1-AP

Synonymes

CMTX, CMTX1, Connexin-32, CX32, Gap junction beta 1 protein, GJB1

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Galerie de données de validation

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  WB analysis of mouse brain using 10450-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10450-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10450-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10450-1-AP

  IP Result of anti-Connexin-32 (IP:10450-1-AP, 4ug; Detection:10450-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (GJB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (GJB1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A375, tissu cutané de souris, tissu hépatique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10450-1-AP cible Connexin-32 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Connexin-32 Protéine recombinante Ag0659
• Nom complet: gap junction protein, beta 1, 32kDa
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002805
• Symbole du gène: GJB1
• Identification du gène (NCBI): 2705
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Connexin-32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-32 is the major gap junction protein of liver, and the expression is also found in brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin-32 plays an important role in peripheral nerve. Defects in Connexin-32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. (PMID: 8266101; 8613761; 8608591; 19265674)

Publications

Species	Application	Title
human	WB	Chemosphere; Organophosphate flame retardant TDCPP: A risk factor for renal cancer?; Authors - Xuan Zhou
human	WB	World J Gastroenterol; PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.; Authors - Xiao-Ming Liu
mouse	WB	Brain Res Bull; Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.; Authors - Chen Men
human	IHC	J Biomed Biotechnol; Ischemia alters the expression of connexins in the aged human brain.; Authors - Nakase Taizen T
human	IHC, IF	Histopathology; Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.; Authors - Wen-Qun Zhong
human	WB	Oncol Lett; Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.; Authors - Lihua Huang

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